RNA interference toward MMP-2 may be an effective therapeutic strategy for cancer
The invasion or metastasis of pancreatic cancer has been known to be a complex process involving many molecular mechanisms, of which proteolytic degradation of extracellular matrix (ECM) exerted by matrix metalloproteinases (MMPs) was considered to be an essential step. Some data suggest that MMP-2 is involved in pancreatic cancer invasion and metastasis, and a high level of MMP-2 has been found to correlate with poor prognosis in patients with pancreatic cancer. Therefore, inhibition of MMP-2 may be of great value in both preventing pancreatic cancer and blocking metastasis of established tumors. A study will be published on March 7, 2009 address the question. The research team led by Professor Song from Department of General Surgery, Affiliated Beijing Tiantan Hospital, Capital Medical University utilized RNA interference system by using the vector (pGPU6)to specifically knock down MMP-2 expression in pancreatic cancer cell. MMP-2 expression was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation, apoptosis were examined by MTT, flow cytometry, respectively. The abilities of adhesion and invasion were detected by cell adhesion assay and cell invasion assay using Transwell chambers.
Data provide evidence that RNA interference against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the pancreatic cancer cells line Bxpc-3, leading to a potent suppression of tumor cells adhesion and invasion without affecting cells proliferation and apoptosis. These findings suggest that RNA interference towards MMP-2 may be an effective therapeutic strategy for the clinical management of pancreatic tumor. Although the leap to clinical practice remains elusive, gene therapy targeting MMP-2 is attractive and warrants further investigation.
Source: World Journal of Gastroenterology
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- 'Personalized' Genome Sequencing Reveals Coding Error In Gene For Inherited Pancreatic Cancerfrom Science DailyThu, 12 Mar 2009, 16:58:28 UTC
- Blood type study sheds light on biology of pancreatic cancerfrom PhysorgWed, 11 Mar 2009, 22:00:27 UTC
- Blood type study sheds light on biology of pancreatic cancerfrom Harvard ScienceTue, 10 Mar 2009, 20:21:15 UTC
- RNA interference toward MMP-2 may be an effective therapeutic strategy for cancerfrom PhysorgMon, 9 Mar 2009, 16:13:31 UTC
- Drinking adds to cancer riskfrom Science AlertSun, 8 Mar 2009, 12:14:08 UTC
- 'Personalized' genome sequencing reveals coding error in gene for inherited pancreatic cancerfrom PhysorgThu, 5 Mar 2009, 19:21:27 UTC
- Two Or More Drinks A Day May Increase Pancreatic Cancer Riskfrom Science DailyThu, 5 Mar 2009, 0:28:21 UTC
- Gene Involved In Pancreatic Cancer Identifiedfrom Science DailyWed, 4 Mar 2009, 2:35:22 UTC
- Pancreatic cancer gene is identifiedfrom UPITue, 3 Mar 2009, 20:14:07 UTC
- Two or more drinks a day may increase pancreatic cancer riskfrom PhysorgTue, 3 Mar 2009, 18:42:41 UTC
- Researchers ID gene involved in pancreatic cancerfrom Science BlogMon, 2 Mar 2009, 22:07:45 UTC
- Researchers ID gene involved in pancreatic cancerfrom PhysorgMon, 2 Mar 2009, 20:14:26 UTC
- Researchers ID gene involved in pancreatic cancerfrom Science BlogMon, 2 Mar 2009, 19:28:20 UTC
- Newly discovered gene plays vital role in cancerfrom PhysorgFri, 27 Feb 2009, 17:36:42 UTC
- Newly discovered gene plays vital role in cancerfrom Science BlogFri, 27 Feb 2009, 15:37:00 UTC