New techniques improve specificity of CRISPR/Cas9 genome editing tools

To overcome the off-target mutations that commonly occur with CRISPR/Cas9 genome editing methods, researchers at Harvard Medical School and Massachusetts General Hospital have developed two strategies that greatly improve the specificity of RNA-guided nucleases for the DNA region targeted to be cut and repaired. A description of these new techniques and their successful use to modify human cancer cells and embryonic stem cells is described in a special issue on genome editing in Human Gene Therapy.

Read the whole article on Physorg

More from Physorg