Gene therapy reverses type 1 diabetes in mice
Researchers have developed an experimental cure for Type 1 diabetes, a disease that affects about one in every 400 to 600 children and adolescents. They will present their results in a mouse model of Type 1 diabetes on Sunday at The Endocrine Society's 92nd Annual Meeting in San Diego. Using gene therapy, the team from Baylor College of Medicine in Houston tried to counter the two defects that cause Type 1 diabetes: autoimmune attack and destruction of the insulin-producing beta cells. They used nonobese diabetic mice, which spontaneously develop diabetes due to autoimmunity, just as humans do with Type 1 diabetes.
"A single treatment cured about 50 percent of the diabetic mice, restoring their blood sugar to normal so that they no longer need insulin injections," said study co-author Lawrence Chan, MD, DSc, chief of Baylor's diabetes, endocrinology and metabolism division.
Type 1 diabetes occurs when the body's immune system attacks and destroys the beta cells in the pancreas, the insulin "factory" of the body. The resulting near-complete deficiency of insulin—the hormone that controls blood sugar—leads to a buildup of high blood sugar and thus diabetes.
In past studies of their original gene therapy, Chan's group was able to stimulate new formation of beta cells in the liver and restore insulin production and normal blood sugar levels in more than 100 mice with chemically induced diabetes. However, in nonobese diabetic mice the treatment failed to reverse Type 1 diabetes because the mouse's immune system killed the newly formed beta cells, he said.
In this research, which was funded by the National Institute of Diabetes, Kidney and Digestive Diseases, Chan said they "added to the original gene therapy approach a protective gene that shields the newly formed beta cells from autoimmune attack." The added gene was for interleukin-10, an important regulator of the immune system. Past studies showed that interleukin-10 can prevent diabetes development in mice but cannot reverse the disease once it has developed because of the lack of beta cells.
However, when the researchers combined the gene therapy with interleukin-10 into a single intravenous injection, the treatment showed a complete reversal of diabetes in half of the mice during more than 20 months' follow-up. Although the therapy did not reverse autoimmunity throughout the body, it protected the new beta cells from the local destructive effect of autoimmunity, Chan explained.
"We developed a protective 'moat' around the new beta cells," he said. "We are now developing other strategies to try to fortify the newly formed beta cells and give them better weapons in addition to the moat, in order to increase the treatment's cure rate."
Why the gene therapy did not work in all the mice is unclear. However, Chan said the treated mice that did not have improvements in their blood sugar did gain weight and lived a little longer than untreated mice.
Source: The Endocrine Society
Articles on the same topic
- Many people with diabetes do not know or heed dangers of hot weatherMon, 21 Jun 2010, 9:36:02 EDT
- Poor control of diabetes may be linked to low vitamin DMon, 21 Jun 2010, 9:35:33 EDT
- Vitamin D deficiency confirmed as common across a range of rheumatic conditionsFri, 18 Jun 2010, 9:04:02 EDT
- Poor control of diabetes may be linked to low vitamin Dfrom Science CentricTue, 22 Jun 2010, 5:32:06 EDT
- Poor control of diabetes may be linked to low vitamin Dfrom PhysorgMon, 21 Jun 2010, 14:01:42 EDT
- Poor control of diabetes may be linked to low vitamin Dfrom Science DailyMon, 21 Jun 2010, 10:30:20 EDT
- Poor control of diabetes may be linked to low vitamin Dfrom Science BlogMon, 21 Jun 2010, 9:30:52 EDT
- Vitamin D deficiency confirmed as common across a range of rheumatic conditionsfrom Science DailyFri, 18 Jun 2010, 11:30:16 EDT
- Vitamin D deficiency confirmed as common across a range of rheumatic conditionsfrom PhysorgFri, 18 Jun 2010, 10:02:02 EDT
- Vitamin D deficiency confirmed as common across a range of rheumatic conditionsfrom Science BlogFri, 18 Jun 2010, 9:01:39 EDT
Latest Science NewsletterGet the latest and most popular science news articles of the week in your Inbox! It's free!
Learn more about
Check out our next project, Biology.Net
From other science news sites
Popular science news articles
- Cell transplant treats Parkinson's in mice under control of designer drug
- Using oxygen to sterilize medical implants could save time and money
- Influence of religion and predestination on evolution and scientific thinking
- Unique fragment from Earth's formation returns after billions of years in cold storage
- Gene therapy shows long-term benefit for treating rare blindness
- Zika present in Americas longer than previously thought
- Hominins may have been food for carnivores 500,000 years ago
- Contamination in North Dakota linked to fracking spills
- Coal-tar based sealcoats on driveways, parking lots far more toxic than suspected
- Hear no evil: Farmed fish found to be hard of hearing
- Team builds first quantum cascade laser on silicon
- Taking aspirin could increase cancer survival by 20 percent
- Mice flown in space show nascent liver damage, says CU Anschutz researcher
- 1.5 C vs 2 C global warming: New study shows why half a degree matters
- Childhood obesity, malnutrition connected to mom's perception of child's weight