Development of a FRET sensor for real-time imaging of intracellular redox dynamics
Tuesday, June 7, 2011 - 15:31
in Physics & Chemistry
A multidisciplinary research team reports a FRET-based biosensor with defined sensitivity and dynamic range for imaging changes in the intracellular redox environment that appear to dictate cell fate. The FRET-based biosensors are a significant advance for routinely measuring oxidative stress in real-time. The sensor is most useful for monitoring intraorganellar glutathione potentials in the relatively high oxidative environments of ER, Golgi and lysosomes.