A shake-up in cell culturing: Flame sterilization may affect the culture

Wednesday, July 1, 2020 - 15:40 in Biology & Nature

Researchers have found that flame-sterilizing shake-flasks, to avoid introducing microbial contaminants, considerably increases the carbon dioxide concentration in the flasks. This enhanced carbon dioxide concentration affects the growth of some microbial species, which may affect the quantity of vaccines or other valuable substances produced by the microbes.

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