Comparing 13 different CRISPR-Cas9 DNA scissors
Friday, June 26, 2020 - 08:41
in Biology & Nature
CRISPR-Cas9 has become one of the most convenient and effective biotechnology tools used to cut specific DNA sequences. Starting from Streptococcus pyogenes Cas9 (SpCas9), a multitude of variants have been engineered and employed for experiments worldwide. Although all these systems are targeting and cleaving a specific DNA sequence, they also exhibit relatively high off-target activities with potentially harmful effects.