Streamlined and scalable CHANGE-Seq method improves understanding of genome editors

Tuesday, June 16, 2020 - 08:20 in Biology & Nature

Scientists at St. Jude Children's Research Hospital have developed an easy to use, sensitive and high-throughput method to define sites of unintended double stranded breaks in DNA caused by genome editors like the CRISPR-Cas9 technique. They called the method Circularization for High-throughput Analysis of Nuclease Genome-wide Effects by Sequencing (CHANGE-seq). The work appears as an advance online publication today in Nature Biotechnology.

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