Longer DNA fragments reveal rare species diversity

A challenge in metagenomics is that the more commonly used sequencing machines generate data in short lengths, while short-read assemblers may not be able to distinguish among multiple occurrences of the same or similar sequences, making it difficult to identify all the members in a microbial community. In the April 2015 issue of Genome Research, a team including DOE JGI researchers compared two ways of using next generation Illumina sequencing machines to help with this.

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