Stem-cell approach shows promise for Duchenne muscular dystrophy
Researchers have shown that transplanting stem cells derived from normal mouse blood vessels into the hearts of mice that model the pathology associated with Duchenne muscular dystrophy (DMD) prevents the decrease in heart function associated with DMD. Their findings appear in the journal Stem Cells Translational Medicine.
Duchenne muscular dystrophy is a genetic disorder caused by a mutation in the gene for dystrophin, a protein that anchors muscle cells in place when they contract. Without dystrophin, muscle contractions tear cell membranes, leading to cell death. The lost muscle cells must be regenerated, but in time, scar tissue replaces the muscle cells, causing the muscle weakness and heart problems typical of DMD.
The U.S. Centers for Disease Control and Prevention estimates that DMD affects one in every 3,500 males. The disease is more prevalent in males because the dystrophin mutation occurs on the X chromosome; males have one X and one Y chromosome, so a male with this mutation will have DMD, while females have two X chromosomes and must have the mutation on both of them to have the disease. Females with the mutation in one X chromosome sometimes develop muscle weakness and heart problems as well, and may pass the mutation on to their children.
Although medical advances have extended the lifespans of DMD patients from their teens or 20s into their early 30s, disease-related damage to the heart and diaphragm still limits their lifespan.
"Almost 100 percent of patients develop dilated cardiomyopathy," in which a weakened heart with enlarged chambers prevents blood from being properly pumped throughout the body, said University of Illinois comparative biosciences professor Suzanne Berry-Miller, who led the study. "Right now, doctors are treating the symptoms of this heart problem by giving patients drugs to try to prolong heart function, but that can't replace the lost or damaged cells," she said.
In the new study, the researchers injected stem cells known as aorta-derived mesoangioblasts (ADM) into the hearts of dystrophin-deficient mice that serve as a model for human DMD. The ADM stem cells have a working copy of the dystrophin gene.
This stem cell therapy prevented or delayed heart problems in mice that did not already show signs of the functional or structural defects typical of Duchenne muscular dystrophy, the researchers report.
Berry-Miller and her colleagues do not yet know why the functional benefits occur, but proposed three potential mechanisms. They observed that some of the injected stem cells became new heart muscle cells that expressed the lacking dystrophin protein. The treatment also caused existing stem cells in the heart to divide and become new heart muscle cells, and the stem cells stimulated new blood vessel formation in the heart. It is not yet clear which of these effects is responsible for delaying the onset of cardiomyopathy, Berry-Miller said.
"These vessel-derived cells might be good candidates for therapy, but the more important thing is the results give us new potential therapeutic targets to study, which may be activated directly without the use of cells that are injected into the patient, such as the ADM in the current study," Berry-Miller said. "Activating stem cells that are already present in the body to repair tissue would avoid the potential requirement to find a match between donors and recipients and potential rejection of the stem cells by the patients."
Despite the encouraging results that show that stem cells yield a functional benefit when administered before pathology arises in DMD mouse hearts, a decline in function was seen in mice that already showed the characteristics of dilated cardiomyopathy. One of these characteristics is the replacement of muscle tissue with connective tissue, known as fibrosis.
This difference may occur, Berry-Miller said, as a result of stem cells landing in a pocket of fibrosis rather than in muscle tissue. The stem cells may then become fibroblasts that generate more connective tissue, increasing the amount of scarring and making heart function worse. This shows that the timing of stem cell insertion plays a crucial role in an increase in heart function in mice lacking the dystrophin protein.
She remains optimistic that these results provide a stepping-stone toward new clinical targets for human DMD patients.
"This is the only study so far where a functional benefit has been observed from stem cells in the dystrophin-deficient heart, or where endogenous stem cells in the heart have been observed to produce new muscle cells that replace those lost in DMD, so I think it opens up a new area to focus on in pre-clinical studies for DMD," Berry-Miller said.
The Illinois Regenerative Medicine Institute supported this research.
- New mouse model reveals a mystery of Duchenne muscular dystrophy, Stanford scientists saySun, 7 Jul 2013, 17:01:22 EDT
- New mouse model for duchenne muscular dystrophy implicates stem cells, Stanford researchers sayThu, 9 Dec 2010, 13:43:04 EST
- Duchenne muscular dystrophy is ultimately a stem cell diseaseThu, 9 Dec 2010, 13:43:07 EST
- New approach to protect the hearts of patients with muscular dystrophyMon, 12 May 2008, 17:21:43 EDT
- While focusing on heart disease, researchers discover new tactic against fatal muscular dystrophySun, 8 Feb 2009, 14:15:36 EST
- Tamoxifen ameliorates symptoms of Duchenne muscular dystrophy, study suggestsfrom Science DailyTue, 15 Jan 2013, 12:50:16 EST
- Tamoxifen helps muscular dystrophy symptoms in micefrom Science BlogTue, 15 Jan 2013, 9:50:42 EST
- Stem-cell approach shows promise for Duchenne muscular dystrophyfrom Science DailyMon, 14 Jan 2013, 20:01:38 EST
- Stem-cell approach shows promise for Duchenne muscular dystrophyfrom Biology News NetMon, 14 Jan 2013, 19:00:47 EST
Latest Science NewsletterGet the latest and most popular science news articles of the week in your Inbox! It's free!
Check out our next project, Biology.Net
From other science news sites
Popular science news articles
- Ocean crust could store many centuries of industrial CO2
- Mysteries of Earth's radiation belts uncovered by NASA twin spacecraft
- Fledgling supernova remnant reveals neutron star's secrets
- CU-Boulder-led team finds first evidence of primates regularly sleeping in caves
- Sea level rise and shoreline changes are lead influences on floods from tropical cyclones
- Stanford study suggests why, in some species, mere presence of males shortens females' lifespan
- 'Spooky action' builds a wormhole between 'entangled' quantum particles
- Fruit flies with better sex lives live longer
- The mystery of neutron stars heats up
- New report calls for attention to abrupt impacts from climate change