How muscle cells seal their membranes
Every cell is enclosed by a thin double layer of lipids that separates the distinct internal environment of the cell from the extracellular space. Damage to this lipid bilayer, also referred to as plasma membrane, disturbs the cellular functions and may lead to the death of the cell. For example, downhill walking tears many little holes into the plasma membranes of the muscle cells in our legs. To prevent irreparable damage, muscle cells have efficient systems to seal these holes again. Researchers at Karlsruhe Institute of Technology (KIT) and Heidelberg University have succeeded for the first time in observing membrane repair in real-time in a living organism. The results were published in the recent issue of the journal Developmental Cell. Using a novel high-resolution imaging method, Prof. Uwe Strähle and Dr. Urmas Roostalu for the first time observed membrane repair in real time in a living animal. They tagged repair proteins with fluorescent proteins in muscle of the transparent zebrafish larvae. With a laser, the researchers burned tiny holes into the plasma membrane of muscle cells and followed the repair of the holes under the microscope. They showed that membrane vesicles together with two proteins Dysferlin and Annexin A6 rapidly form a repair patch. Other Annexins accumulate subsequently on the injured membrane. These studies by Karlsruhe and Heidelberg researchers suggest that the cell assembles a multilayered repair patch from the inside that seals off the cell's interior from the extracellular environment. Moreover, it was found that there is a specialized membrane area that supplies rapidly the membrane that is needed for sealing the plasma membrane hole.
This animal model for membrane repair will contribute to the identification of new proteins in this sealing process and will help elucidate the underlying mechanisms. The results may contribute to the development of therapies for human myopathies and open up new possibilities in biotechnology.
Latest Science NewsletterGet the latest and most popular science news articles of the week in your Inbox! It's free!
Learn more about
Check out our next project, Biology.Net
From other science news sites
Popular science news articles
- Cloth masks offer poor protection against air pollution
- Squid, jellyfish and wrinkled skin inspire materials for anti-glare screens and encryption
- Fungi recycle rechargeable lithium-ion batteries
- NASA sees Tropical Storm Fiona weakening from wind shear
- Why prisons continue to grow, even when crime declines
- Paraplegics regain some feeling, movement after using brain-machine interfaces
- In right balance, environmental regulations increased firms' profits, new study finds
- Caught in the act: First videos of a coral's bleaching behavior
- Discovery of a unique subcellular structure determining the orientation of cell division
- 'Chemtrails' not real, say leading atmospheric science experts