Written in Red: Red-Emitting Dyes for Optical Microscopy and Nanoscopy
Wednesday, July 7, 2010 - 09:21
in Physics & Chemistry
(PhysOrg.com) -- Far-field optical nanoscopy methods, especially STED (stimulated emission depletion), pose very strict and, at times, contradictory requirements on the utilized fluorescent markers. Photostable fluorescent dyes that absorb in the red optical region are indispensable as labels for various micro- and nanoscopic studies (e.g., with commercially available STED microscopes). Despite many attempts to design novel and improved red-emitting dyes, the number of compounds that perform satisfactorily in fluorescence-based microscopy is still limited.