Seeing the Brain's Circuits with a New Clarity

For scientists working to uncover the mysteries of the brain, fat is a problem. The fats inside cells bend and scatter light, obscuring researchers' views when they try to peer deep into tissue. A new technique developed by HHMI scientists solves that problem by removing the fat from the brain and supporting the remaining brain structures in a hydrogel--literally giving scientists a clear view of an intact brain. The technique, called CLARITY, transforms biological tissue into an optically transparent sample that retains its original structure and molecular information.

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