Shortening guide RNA markedly improves specificity of CRISPR-Cas nucleases
Monday, January 27, 2014 - 13:00
in Biology & Nature
A simple adjustment to a powerful gene-editing tool may be able to improve its specificity. Investigators have found that adjusting the length of the the guide RNA component of the synthetic enzymes called CRISPR-Cas RNA-guided nucleases can substantially reduce the occurrence of off-target DNA mutations.