Scutellarin From Tomatoes? Vacuum Method Powers Synthetic Biology in Fruit
Researchers have developed an improved vacuum-infiltration system that overcomes long-standing barriers in tomato genetic engineering. This innovative system enables high-efficiency Agrobacterium-mediated transient expression in both tomato leaves and, for the first time, attached fruits. The system facilitates rapid gene functional analysis and element screening, dramatically shortening experimental timelines. Key outcomes include successful expression of multiple reporter genes, identification of trichome-specific promoters, production of flavonoid-rich tomato lines, and synthesis of the high-value compound scutellarin. By enhancing the speed, reliability, and versatility of gene expression studies, this new method advances both fundamental research and synthetic biology applications in tomato.