New imaging approach accurately measures lipid and metabolite distributions in biological samples
Wednesday, July 30, 2014 - 06:30
in Biology & Nature
To understand how cells converse, scientists at Pacific Northwest National Laboratory and Oregon Health & Science University designed an approach that accurately determines the spatial location of molecules and quantifies lipids and metabolites in biological samples. The new approach efficiently accounts for signal suppression or matrix effect (see sidebar) that may significantly alter molecules' distributions obtained in mass spectrometry imaging experiments. Compensation for matrix effects is achieved by adding appropriate internal standards to the solvent used in nano-DESI imaging.