An alternative TALEN/CRISPR-mediated gene insertion technique described in detail

Friday, December 18, 2015 - 05:40 in Biology & Nature

Green spots observed in cells indicate successful insertion of the foreign fluorescent protein gene by the PITCh system. A streamlined protocol for an alternative gene insertion method using genome editing technologies, the PITCh (Precise Integration into Target Chromosome) system, has been reported in Nature Protocols by Specially Appointed Lecturer Tetsushi Sakuma, Professor Takashi Yamamoto, Specially Appointed Associate Professor Ken-Ichi T Suzuki, and their colleagues at Hiroshima University, Japan.

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