Highly efficient CRISPR knock-in in mouse

Friday, May 1, 2015 - 18:13 in Biology & Nature

This image shows a schematic diagram of the highly efficient CRISPR/Cas system, which leads to super efficient targeted insertion (knock-in) of a long donor insert into mouse genome. Genome editing using CRISPR/Cas system has enabled direct modification of the mouse genome in fertilized mouse eggs, leading to rapid, convenient, and efficient one-step production of knockout mice without embryonic stem cells. In contrast to the ease of targeted gene deletion, the complementary application, called targeted gene cassette insertion or knock-in, in fertilized mouse eggs by CRISPR/Cas mediated genome editing still remains a tough challenge.

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